Part:BBa_K562000:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

User Reviews

UNIQ5b7c871db468a21c-partinfo-00000000-QINU UNIQ5b7c871db468a21c-partinfo-00000001-QINU

••••• iGEM Dundee 2011

This part was seen work in practice. It is a published consitutive promoter, and we have shown it results in eventual protein production for numerous targets. See BBa_K562009 for the most detailed characterisation.

• iGEM Dundee 2012

Although this part was seen work in practice, the Dundee 2012 team found that the rookie Dundee 2011 team had make some mistakes in their cloning. BBa_K562000 was found not to have been cloned to Biobrick [10] standard as the correct prefix and suffix sequences were not used. BBa_K562000 is now replaced by BBa_K895000.

Results

A gene encoding an engineered mCherry protein was placed downstream of the tat promoter from BBa_K562000 and E. coli was transformed. mCherry fluorescence was observed by confocal microscopy as shown in Figure 1.

Figure 1: Transcription and translation of mCherry driven by the E. coli tat promoter from BBa_K562000.

UNIQ5b7c871db468a21c-partinfo-0000000A-QINU